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After undergoing extensive testing, 300 nm light was selected as a reference wavelength for comparison with the other wavelengths. The ratios of wavelength dependent CE [CD] values for the four wavelengths are shown in Figure 3. As can be seen from Figure 3, the wavelengths of the optimum CE values are close, but they are not identical. In this work, a 660 nm laser diode [LD] was used as the illumination source. The transmission properties of the tissue are mainly determined by absorption by melanin, hemoglobin, and water. (17, 18) The absorption spectra of melanin and hemoglobin have their maximum absorption in the visible wavelength range [780 to 900 nm] (19, 20) as shown in Figure 4. As can be seen from Figure 4, there are other absorption peaks for melanin and hemoglobin, but they do not have an effect on the light transmittance to any significant extent. The transmission spectra for the water solution were obtained from UV-vis spectrophotometer. The absorption spectra of water and hemoglobin exhibit two prominent peaks at the wavelengths of 400 and 540 nm [wavelengths at which hemoglobin has its maximum absorption] (21, 22). The wavelength peaks of the two proteins are located close to each other and thus do not affect the transmission of light. As shown in Figure 5, the ratio of the absorption spectra of the melanin solution and the cytoplasm of the cells, measured at 780 nm, is shown on the vertical axis and the absorption of the cytoplasm is shown on the horizontal axis. It can be seen that the absorption of the cytoplasm is much higher than the absorption of the melanin. Thus, the concentration of the melanin in the cytoplasm is much lower than that of the hemoglobin in the cytoplasm. The ratio of the absorption spectra of the hemoglobin and the water solution are plotted in Figure 6, and it can be seen that the maximum absorption for hemoglobin is around 540 nm. Therefore, the wavelength range for the optimum CE value is around 720 nm. (The optimum CE for the 660 nm wavelength is not shown in Figure 6). Thus, it can be concluded that the CE that is measured for a particular wavelength is influenced by both the absorption of melanin and water, as well as the water absorption in the cytoplasm. However, the ratio of the cytoplasm to the melanin concentration that is measured at 780 nm is much greater than the
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Download Maulid Diba Pdf To Word 1
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