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Urokinase-type plasminogen activator (u-PA) and plasmin are proteolytic enzymes of which the expression in human melanoma cells and their activity toward extracellular matrix proteins are unknown. The presence and activity of u-PA were investigated in two human melanoma cell lines, WM115 and WM266-4, using immunodetection and a specific activity assay for u-PA. A 3.5-fold higher concentration of u-PA was present in the culture medium of the high-metastatic cell line, WM266-4, than in the medium
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