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To determine whether extracellular neurotransmitters could influence lactate production from pyruvate, the effects of N-methyl-D-aspartate (NMDA), an excitatory transmitter, on lactate production by cultured cortical astrocytes were investigated. Lactate production was measured using [1-14C]pyruvate as substrate in the presence of beta-NAD+, nicotinamide, and rotenone. NMDA, 50 micromol/l, concentration-dependently inhibited lactate production by cortical astrocytes. The inhibitory effect was blocked by tetrodotoxin, indicating that the effect was mediated via presynaptic non-NMDA receptors. The incubation of these cells with 1 mmol/l NMDA, concentration that activated non-NMDA receptors but which did not affect lactate production, also inhibited lactate production. The effect was attenuated by 6-cyano-7-nitro-quinoxaline-2,3-dione. The inhibition was also produced by D,L-2-amino-5-phosphonopentanoic acid, an N-methyl-D-aspartate receptor antagonist, and by kainate. The results suggest that NMDA inhibits lactate production from pyruvate in cultured astrocytes, probably via non-NMDA receptors on the pre-synaptic terminals.In response to the recent demand from National Parks Foundation for me to do a “Travels with Liz” story, it’s with great pleasure I speak to you from my parlor room in Taos, New Mexico.
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